Hydrolysis of beta-1,3/1,6-glucan by glycoside hydrolase family 16 endo-1,3(4)-beta-glucanase from the basidiomycete Phanerochaete chrysosporium.
Identifieur interne : 000773 ( Main/Exploration ); précédent : 000772; suivant : 000774Hydrolysis of beta-1,3/1,6-glucan by glycoside hydrolase family 16 endo-1,3(4)-beta-glucanase from the basidiomycete Phanerochaete chrysosporium.
Auteurs : Rie Kawai [Japon] ; Kiyohiko Igarashi ; Makoto Yoshida ; Motomitsu Kitaoka ; Masahiro SamejimaSource :
- Applied microbiology and biotechnology [ 0175-7598 ] ; 2006.
Descripteurs français
- KwdFr :
- ADN complémentaire (composition chimique), ADN complémentaire (génétique), Alignement de séquences (MeSH), Analyse de séquence d'ADN (MeSH), Cellulase (génétique), Cellulase (isolement et purification), Cellulase (métabolisme), Chromatographie sur couche mince (méthodes), Clonage moléculaire (méthodes), Données de séquences moléculaires (MeSH), Glycosidases (génétique), Glycosidases (isolement et purification), Glycosidases (métabolisme), Hydrolyse (MeSH), Phanerochaete (enzymologie), Phanerochaete (génétique), Phanerochaete (métabolisme), Pichia (génétique), Protéines fongiques (génétique), Protéines fongiques (isolement et purification), Protéines fongiques (métabolisme), Similitude de séquences d'acides aminés (MeSH), Structure moléculaire (MeSH), Séquence d'acides aminés (MeSH), Séquence glucidique (MeSH), Séquence nucléotidique (MeSH), bêta-Glucanes (composition chimique), bêta-Glucanes (métabolisme), Électrophorèse sur gel de polyacrylamide (méthodes).
- MESH :
- composition chimique : ADN complémentaire, bêta-Glucanes.
- enzymologie : Phanerochaete.
- génétique : ADN complémentaire, Cellulase, Glycosidases, Phanerochaete, Pichia, Protéines fongiques.
- isolement et purification : Cellulase, Glycosidases, Protéines fongiques.
- métabolisme : Cellulase, Glycosidases, Phanerochaete, Protéines fongiques, bêta-Glucanes.
- méthodes : Chromatographie sur couche mince, Clonage moléculaire, Électrophorèse sur gel de polyacrylamide.
- Alignement de séquences, Analyse de séquence d'ADN, Données de séquences moléculaires, Hydrolyse, Similitude de séquences d'acides aminés, Structure moléculaire, Séquence d'acides aminés, Séquence glucidique, Séquence nucléotidique.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Base Sequence (MeSH), Carbohydrate Sequence (MeSH), Cellulase (genetics), Cellulase (isolation & purification), Cellulase (metabolism), Chromatography, Thin Layer (methods), Cloning, Molecular (methods), DNA, Complementary (chemistry), DNA, Complementary (genetics), Electrophoresis, Polyacrylamide Gel (methods), Fungal Proteins (genetics), Fungal Proteins (isolation & purification), Fungal Proteins (metabolism), Glycoside Hydrolases (genetics), Glycoside Hydrolases (isolation & purification), Glycoside Hydrolases (metabolism), Hydrolysis (MeSH), Molecular Sequence Data (MeSH), Molecular Structure (MeSH), Phanerochaete (enzymology), Phanerochaete (genetics), Phanerochaete (metabolism), Pichia (genetics), Sequence Alignment (MeSH), Sequence Analysis, DNA (MeSH), Sequence Homology, Amino Acid (MeSH), beta-Glucans (chemistry), beta-Glucans (metabolism).
- MESH :
- chemical , chemistry : DNA, Complementary, beta-Glucans.
- chemical , genetics : Cellulase, DNA, Complementary, Fungal Proteins, Glycoside Hydrolases.
- chemical , isolation & purification : Cellulase, Fungal Proteins, Glycoside Hydrolases.
- chemical , metabolism : Cellulase, Fungal Proteins, Glycoside Hydrolases, beta-Glucans.
- enzymology : Phanerochaete.
- genetics : Phanerochaete, Pichia.
- metabolism : Phanerochaete.
- methods : Chromatography, Thin Layer, Cloning, Molecular, Electrophoresis, Polyacrylamide Gel.
- Amino Acid Sequence, Base Sequence, Carbohydrate Sequence, Hydrolysis, Molecular Sequence Data, Molecular Structure, Sequence Alignment, Sequence Analysis, DNA, Sequence Homology, Amino Acid.
Abstract
When Phanerochaete chrysosporium was grown with laminarin (a beta-1,3/1,6-glucan) as the sole carbon source, a beta-1,3-glucanase with a molecular mass of 36 kDa was produced as a major extracellular protein. The cDNA encoding this enzyme was cloned, and the deduced amino acid sequence revealed that this enzyme belongs to glycoside hydrolase family 16; it was named Lam16A. Recombinant Lam16A, expressed in the methylotrophic yeast Pichia pastoris, randomly hydrolyzes linear beta-1,3-glucan, branched beta-1,3/1,6-glucan, and beta-1,3-1,4-glucan, suggesting that the enzyme is a typical endo-1,3(4)-beta-glucanase (EC 3.2.1.6) with broad substrate specificity for beta-1,3-glucans. When laminarin and lichenan were used as substrates, Lam16A produced 6-O-glucosyl-laminaritriose (beta-D-Glcp-(1->6)-beta-D-Glcp-(1->3)-beta-D-Glcp-(1->3)-D-Glc) and 4-O-glucosyl-laminaribiose (beta-D-Glcp-(1->4)-beta-D-Glcp-(1->3)-D-Glc), respectively, as one of the major products. These results suggested that the enzyme strictly recognizes beta-D-Glcp-(1->3)-D-Glcp at subsites -2 and -1, whereas it permits 6-O-glucosyl substitution at subsite +1 and a beta-1,4-glucosidic linkage at the catalytic site. Consequently, Lam16A generates non-branched oligosaccharide from branched beta-1,3/1,6-glucan and, thus, may contribute to the effective degradation of such molecules in combination with other extracellular beta-1,3-glucanases.
DOI: 10.1007/s00253-005-0214-4
PubMed: 16374635
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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<author><name sortKey="Yoshida, Makoto" sort="Yoshida, Makoto" uniqKey="Yoshida M" first="Makoto" last="Yoshida">Makoto Yoshida</name>
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<author><name sortKey="Samejima, Masahiro" sort="Samejima, Masahiro" uniqKey="Samejima M" first="Masahiro" last="Samejima">Masahiro Samejima</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Hydrolysis of beta-1,3/1,6-glucan by glycoside hydrolase family 16 endo-1,3(4)-beta-glucanase from the basidiomycete Phanerochaete chrysosporium.</title>
<author><name sortKey="Kawai, Rie" sort="Kawai, Rie" uniqKey="Kawai R" first="Rie" last="Kawai">Rie Kawai</name>
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<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Biomaterials Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657</wicri:regionArea>
<orgName type="university">Université de Tokyo</orgName>
<placeName><settlement type="city">Tokyo</settlement>
<region type="province">Région de Kantō</region>
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<author><name sortKey="Igarashi, Kiyohiko" sort="Igarashi, Kiyohiko" uniqKey="Igarashi K" first="Kiyohiko" last="Igarashi">Kiyohiko Igarashi</name>
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<series><title level="j">Applied microbiology and biotechnology</title>
<idno type="ISSN">0175-7598</idno>
<imprint><date when="2006" type="published">2006</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term>
<term>Base Sequence (MeSH)</term>
<term>Carbohydrate Sequence (MeSH)</term>
<term>Cellulase (genetics)</term>
<term>Cellulase (isolation & purification)</term>
<term>Cellulase (metabolism)</term>
<term>Chromatography, Thin Layer (methods)</term>
<term>Cloning, Molecular (methods)</term>
<term>DNA, Complementary (chemistry)</term>
<term>DNA, Complementary (genetics)</term>
<term>Electrophoresis, Polyacrylamide Gel (methods)</term>
<term>Fungal Proteins (genetics)</term>
<term>Fungal Proteins (isolation & purification)</term>
<term>Fungal Proteins (metabolism)</term>
<term>Glycoside Hydrolases (genetics)</term>
<term>Glycoside Hydrolases (isolation & purification)</term>
<term>Glycoside Hydrolases (metabolism)</term>
<term>Hydrolysis (MeSH)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Molecular Structure (MeSH)</term>
<term>Phanerochaete (enzymology)</term>
<term>Phanerochaete (genetics)</term>
<term>Phanerochaete (metabolism)</term>
<term>Pichia (genetics)</term>
<term>Sequence Alignment (MeSH)</term>
<term>Sequence Analysis, DNA (MeSH)</term>
<term>Sequence Homology, Amino Acid (MeSH)</term>
<term>beta-Glucans (chemistry)</term>
<term>beta-Glucans (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire (composition chimique)</term>
<term>ADN complémentaire (génétique)</term>
<term>Alignement de séquences (MeSH)</term>
<term>Analyse de séquence d'ADN (MeSH)</term>
<term>Cellulase (génétique)</term>
<term>Cellulase (isolement et purification)</term>
<term>Cellulase (métabolisme)</term>
<term>Chromatographie sur couche mince (méthodes)</term>
<term>Clonage moléculaire (méthodes)</term>
<term>Données de séquences moléculaires (MeSH)</term>
<term>Glycosidases (génétique)</term>
<term>Glycosidases (isolement et purification)</term>
<term>Glycosidases (métabolisme)</term>
<term>Hydrolyse (MeSH)</term>
<term>Phanerochaete (enzymologie)</term>
<term>Phanerochaete (génétique)</term>
<term>Phanerochaete (métabolisme)</term>
<term>Pichia (génétique)</term>
<term>Protéines fongiques (génétique)</term>
<term>Protéines fongiques (isolement et purification)</term>
<term>Protéines fongiques (métabolisme)</term>
<term>Similitude de séquences d'acides aminés (MeSH)</term>
<term>Structure moléculaire (MeSH)</term>
<term>Séquence d'acides aminés (MeSH)</term>
<term>Séquence glucidique (MeSH)</term>
<term>Séquence nucléotidique (MeSH)</term>
<term>bêta-Glucanes (composition chimique)</term>
<term>bêta-Glucanes (métabolisme)</term>
<term>Électrophorèse sur gel de polyacrylamide (méthodes)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Complementary</term>
<term>beta-Glucans</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Cellulase</term>
<term>DNA, Complementary</term>
<term>Fungal Proteins</term>
<term>Glycoside Hydrolases</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Cellulase</term>
<term>Fungal Proteins</term>
<term>Glycoside Hydrolases</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cellulase</term>
<term>Fungal Proteins</term>
<term>Glycoside Hydrolases</term>
<term>beta-Glucans</term>
</keywords>
<keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>ADN complémentaire</term>
<term>bêta-Glucanes</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Phanerochaete</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Phanerochaete</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Phanerochaete</term>
<term>Pichia</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN complémentaire</term>
<term>Cellulase</term>
<term>Glycosidases</term>
<term>Phanerochaete</term>
<term>Pichia</term>
<term>Protéines fongiques</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Cellulase</term>
<term>Glycosidases</term>
<term>Protéines fongiques</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Phanerochaete</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Chromatography, Thin Layer</term>
<term>Cloning, Molecular</term>
<term>Electrophoresis, Polyacrylamide Gel</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cellulase</term>
<term>Glycosidases</term>
<term>Phanerochaete</term>
<term>Protéines fongiques</term>
<term>bêta-Glucanes</term>
</keywords>
<keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Chromatographie sur couche mince</term>
<term>Clonage moléculaire</term>
<term>Électrophorèse sur gel de polyacrylamide</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Base Sequence</term>
<term>Carbohydrate Sequence</term>
<term>Hydrolysis</term>
<term>Molecular Sequence Data</term>
<term>Molecular Structure</term>
<term>Sequence Alignment</term>
<term>Sequence Analysis, DNA</term>
<term>Sequence Homology, Amino Acid</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Alignement de séquences</term>
<term>Analyse de séquence d'ADN</term>
<term>Données de séquences moléculaires</term>
<term>Hydrolyse</term>
<term>Similitude de séquences d'acides aminés</term>
<term>Structure moléculaire</term>
<term>Séquence d'acides aminés</term>
<term>Séquence glucidique</term>
<term>Séquence nucléotidique</term>
</keywords>
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<front><div type="abstract" xml:lang="en">When Phanerochaete chrysosporium was grown with laminarin (a beta-1,3/1,6-glucan) as the sole carbon source, a beta-1,3-glucanase with a molecular mass of 36 kDa was produced as a major extracellular protein. The cDNA encoding this enzyme was cloned, and the deduced amino acid sequence revealed that this enzyme belongs to glycoside hydrolase family 16; it was named Lam16A. Recombinant Lam16A, expressed in the methylotrophic yeast Pichia pastoris, randomly hydrolyzes linear beta-1,3-glucan, branched beta-1,3/1,6-glucan, and beta-1,3-1,4-glucan, suggesting that the enzyme is a typical endo-1,3(4)-beta-glucanase (EC 3.2.1.6) with broad substrate specificity for beta-1,3-glucans. When laminarin and lichenan were used as substrates, Lam16A produced 6-O-glucosyl-laminaritriose (beta-D-Glcp-(1->6)-beta-D-Glcp-(1->3)-beta-D-Glcp-(1->3)-D-Glc) and 4-O-glucosyl-laminaribiose (beta-D-Glcp-(1->4)-beta-D-Glcp-(1->3)-D-Glc), respectively, as one of the major products. These results suggested that the enzyme strictly recognizes beta-D-Glcp-(1->3)-D-Glcp at subsites -2 and -1, whereas it permits 6-O-glucosyl substitution at subsite +1 and a beta-1,4-glucosidic linkage at the catalytic site. Consequently, Lam16A generates non-branched oligosaccharide from branched beta-1,3/1,6-glucan and, thus, may contribute to the effective degradation of such molecules in combination with other extracellular beta-1,3-glucanases.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16374635</PMID>
<DateCompleted><Year>2006</Year>
<Month>10</Month>
<Day>10</Day>
</DateCompleted>
<DateRevised><Year>2006</Year>
<Month>11</Month>
<Day>15</Day>
</DateRevised>
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<Issue>6</Issue>
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<Month>Aug</Month>
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<Title>Applied microbiology and biotechnology</Title>
<ISOAbbreviation>Appl Microbiol Biotechnol</ISOAbbreviation>
</Journal>
<ArticleTitle>Hydrolysis of beta-1,3/1,6-glucan by glycoside hydrolase family 16 endo-1,3(4)-beta-glucanase from the basidiomycete Phanerochaete chrysosporium.</ArticleTitle>
<Pagination><MedlinePgn>898-906</MedlinePgn>
</Pagination>
<Abstract><AbstractText>When Phanerochaete chrysosporium was grown with laminarin (a beta-1,3/1,6-glucan) as the sole carbon source, a beta-1,3-glucanase with a molecular mass of 36 kDa was produced as a major extracellular protein. The cDNA encoding this enzyme was cloned, and the deduced amino acid sequence revealed that this enzyme belongs to glycoside hydrolase family 16; it was named Lam16A. Recombinant Lam16A, expressed in the methylotrophic yeast Pichia pastoris, randomly hydrolyzes linear beta-1,3-glucan, branched beta-1,3/1,6-glucan, and beta-1,3-1,4-glucan, suggesting that the enzyme is a typical endo-1,3(4)-beta-glucanase (EC 3.2.1.6) with broad substrate specificity for beta-1,3-glucans. When laminarin and lichenan were used as substrates, Lam16A produced 6-O-glucosyl-laminaritriose (beta-D-Glcp-(1->6)-beta-D-Glcp-(1->3)-beta-D-Glcp-(1->3)-D-Glc) and 4-O-glucosyl-laminaribiose (beta-D-Glcp-(1->4)-beta-D-Glcp-(1->3)-D-Glc), respectively, as one of the major products. These results suggested that the enzyme strictly recognizes beta-D-Glcp-(1->3)-D-Glcp at subsites -2 and -1, whereas it permits 6-O-glucosyl substitution at subsite +1 and a beta-1,4-glucosidic linkage at the catalytic site. Consequently, Lam16A generates non-branched oligosaccharide from branched beta-1,3/1,6-glucan and, thus, may contribute to the effective degradation of such molecules in combination with other extracellular beta-1,3-glucanases.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kawai</LastName>
<ForeName>Rie</ForeName>
<Initials>R</Initials>
<AffiliationInfo><Affiliation>Department of Biomaterials Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Igarashi</LastName>
<ForeName>Kiyohiko</ForeName>
<Initials>K</Initials>
</Author>
<Author ValidYN="Y"><LastName>Yoshida</LastName>
<ForeName>Makoto</ForeName>
<Initials>M</Initials>
</Author>
<Author ValidYN="Y"><LastName>Kitaoka</LastName>
<ForeName>Motomitsu</ForeName>
<Initials>M</Initials>
</Author>
<Author ValidYN="Y"><LastName>Samejima</LastName>
<ForeName>Masahiro</ForeName>
<Initials>M</Initials>
</Author>
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<Language>eng</Language>
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<AccessionNumberList><AccessionNumber>AB092837</AccessionNumber>
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<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
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<ArticleDate DateType="Electronic"><Year>2005</Year>
<Month>12</Month>
<Day>23</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>Germany</Country>
<MedlineTA>Appl Microbiol Biotechnol</MedlineTA>
<NlmUniqueID>8406612</NlmUniqueID>
<ISSNLinking>0175-7598</ISSNLinking>
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<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D018076">DNA, Complementary</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D047071">beta-Glucans</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>37361-00-5</RegistryNumber>
<NameOfSubstance UI="C064197">beta-1,6-glucan</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>9051-97-2</RegistryNumber>
<NameOfSubstance UI="C033363">beta-1,3-glucan</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.2.1.-</RegistryNumber>
<NameOfSubstance UI="D006026">Glycoside Hydrolases</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.2.1.4</RegistryNumber>
<NameOfSubstance UI="D002480">Cellulase</NameOfSubstance>
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<MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002240" MajorTopicYN="N">Carbohydrate Sequence</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002480" MajorTopicYN="N">Cellulase</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002855" MajorTopicYN="N">Chromatography, Thin Layer</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018076" MajorTopicYN="N">DNA, Complementary</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004591" MajorTopicYN="N">Electrophoresis, Polyacrylamide Gel</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005656" MajorTopicYN="N">Fungal Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006026" MajorTopicYN="N">Glycoside Hydrolases</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006868" MajorTopicYN="N">Hydrolysis</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015394" MajorTopicYN="N">Molecular Structure</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020075" MajorTopicYN="N">Phanerochaete</DescriptorName>
<QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010843" MajorTopicYN="N">Pichia</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016415" MajorTopicYN="N">Sequence Alignment</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017386" MajorTopicYN="N">Sequence Homology, Amino Acid</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D047071" MajorTopicYN="N">beta-Glucans</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2005</Year>
<Month>09</Month>
<Day>08</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2005</Year>
<Month>10</Month>
<Day>10</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="revised"><Year>2005</Year>
<Month>10</Month>
<Day>07</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2005</Year>
<Month>12</Month>
<Day>24</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2006</Year>
<Month>10</Month>
<Day>13</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2005</Year>
<Month>12</Month>
<Day>24</Day>
<Hour>9</Hour>
<Minute>0</Minute>
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</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">16374635</ArticleId>
<ArticleId IdType="doi">10.1007/s00253-005-0214-4</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Japon</li>
</country>
<region><li>Région de Kantō</li>
</region>
<settlement><li>Tokyo</li>
</settlement>
<orgName><li>Université de Tokyo</li>
</orgName>
</list>
<tree><noCountry><name sortKey="Igarashi, Kiyohiko" sort="Igarashi, Kiyohiko" uniqKey="Igarashi K" first="Kiyohiko" last="Igarashi">Kiyohiko Igarashi</name>
<name sortKey="Kitaoka, Motomitsu" sort="Kitaoka, Motomitsu" uniqKey="Kitaoka M" first="Motomitsu" last="Kitaoka">Motomitsu Kitaoka</name>
<name sortKey="Samejima, Masahiro" sort="Samejima, Masahiro" uniqKey="Samejima M" first="Masahiro" last="Samejima">Masahiro Samejima</name>
<name sortKey="Yoshida, Makoto" sort="Yoshida, Makoto" uniqKey="Yoshida M" first="Makoto" last="Yoshida">Makoto Yoshida</name>
</noCountry>
<country name="Japon"><region name="Région de Kantō"><name sortKey="Kawai, Rie" sort="Kawai, Rie" uniqKey="Kawai R" first="Rie" last="Kawai">Rie Kawai</name>
</region>
</country>
</tree>
</affiliations>
</record>
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